摘要
利用受精卵原核的显微注射技术 ,将人类HLA Ⅱ类DRα及DRB1 0 4 0 1两种基因 ,显微注射至C5 7BL 6×DBA 1杂交小鼠受精卵中 ;并移植至假孕受体鼠的输卵管内。实验先后有 8只鼠妊娠 ,稳定遗传五代 ,经PCR检测 95只HLA DRα、DRB1 0 4 0 1阳性。α 32P dCTP斑点杂交与Southern印迹杂交鉴定出 6 8只整合含有DRα及DRB1 0 4 0 1混合型的转基因小鼠 ,有效率为 2 0 .9%。经Northern杂交[1] 和RT PCR检测 ,其HLA DRα、DRB1 0 4 0 1基因在脾脏和肾脏中均有表达。结果说明 ,携带人类HLA Ⅱ类DRα和DRB1 0 4 0 1基因的转基因动物模型构建成功。
HLA\|DR α and DRB^10401 transgenic mice model was constructed by pronucleus microinjection technique.The DR α and DRB\-1\{\}\+*0401 were microinjection into the fertilized eggs of C57BL/6×DBA/1 F1 mice.The total integrational rate and efficient rate of transgene were 29.1% and 20.9% assessed by PCR,Dot blot and southern blot,respectively at the same time,These results demonstrated that HLA\|DR4 molecule participanted in murine immune regulation.It can enhance our understanding of the pathophysiological rule of human MHC\|Ⅱ molecules,although its association with disease remains poorly understood,greatly benefit to the study of human autoimmune disease.\;
出处
《中国生物工程杂志》
CAS
CSCD
2002年第3期64-67,共4页
China Biotechnology
基金
国家自然科学基金资助课题
编号 39370 865
