摘要
目的 研究氯胺酮、丙泊酚在体外对佛波酯 (PMA)刺激后人中性粒细胞 (PMN)呼吸爆发功能的影响。方法 采健康人静脉血 (n =5 ) ,分为对照组 (C组 ) ,氯胺酮 1、2、3组 (K1、K2、K3组 ) ,丙泊酚 1、2、3组 (P1、P2、P3组 ) ,氯胺酮、丙泊酚终浓度分别为 0、3、30、30 0 μg/ml和 5、5 0、5 0 0 μg/ml,以全血法测定PMN呼吸爆发强度 (DHR标记 ,流式细胞法 )。 结果 氯胺酮、丙泊酚均可剂量依赖性地抑制PMA刺激后PMN的呼吸爆发强度 ,P2、K3、P3组呼吸爆发强度均非常显著地低于C组 (P <0 0 1) ,丙泊酚的抑制效应又强于对应浓度的氯胺酮 (P2vsK2 ,P <0 0 5 ;P3vsK3,P <0 0 1)。结论 氯胺酮、丙泊酚抑制PMN呼吸爆发的作用 ,可能会影响到围术期病人的免疫状态 。
Objective To investigate the effects of ketamine or propofol on the respiratory burst of PMA-stimulated human neutrophils in vitro.Methods Blood samples were collected from healthy volunteers(n=5).Groups were designed as control,ketamine 1-3(K 1-K 3) and propofol 1-3(P 1-P 3).The final concentrations of ketamine or propofol were 0,3,30,300μg/ml or 5,50,500μg/ml respectively.Respiratory burst was analyzed with whole blood by flow cytometry with dihydrorhodamine 123 as fluorescent marker.Results Both ketamine and propofol dose-dependently inhibited the respiratory burst of PMA-stimulated human neutrophils in vitro.Compared with group C,the values of P 2,K 3 and P 3 were significantly lower (P<0.01).Meanwhile,the inhibitive effect of propofol was stronger than that of ketamine groups(P 2 vs K 2,P<0.05;P 3 vs K 3,P<0.01).Conclusion The inhibitive effects of ketamine or propofol on the respiratory burst of neutrophils may affect the immunological condition of the peri-operative patients.
出处
《临床麻醉学杂志》
CAS
CSCD
2002年第7期367-368,共2页
Journal of Clinical Anesthesiology
