摘要
目的 通过检测乙酰胆碱对蒙古沙鼠耳蜗中一氧化氮合酶 (nitricoxidesynthase ,NOS)的影响 ,初步探讨耳蜗中NOS的调节机制。方法 蒙古沙鼠耳蜗经还原型辅酶Ⅱ -黄递酶 (NADPH -黄递酶 )染色后行耳蜗铺片 ,分别以同一动物两侧耳蜗为实验组和对照组进行对比观察 ,检测乙酰胆碱 (ACh)对耳蜗NOS活性的影响 ,其染色深度的差异通过HPIAS - 10 0 0高清晰度彩色病理图像免疫组化测量系统判断。结果 蒙古沙鼠耳蜗外毛细胞基底部传出神经末梢呈现NADPH -黄递酶染色阳性反应 ,ACh处理后染色深度明显增强 ,其平均灰度值从 74.2 8± 11.71减低至 46 .5± 7.78(P <0 .0 1)。结论 蒙古沙鼠耳蜗外毛细胞基底部的传出神经末梢可见NOS分布 ,ACh能增强其活性 。
Objective To investigate the influence of acetylcholine (ACh) on the activity of NOS so as to primarily elucidate the regulating mechanism of NOS. Methods After stained with NADPH-diaphorase, surface preparation of Mongolian gerbil cochleae was performed. For evaluating the influence of ACh on the activities of NOS, the depth of NADPH-diaphorase staining in both cochleae of the same animal, which was measured with High Resolution Image Analysis System (HPIAS-1000), was compared, one for ACh-treated group and another for control.Results The NADPH-diaphorase staining was labeled in the efferent endings at base of outer hair cells (OHCs). After ACh treatment, the depth of the HADPH-diaphorase staining significantly increased, showing that the average gray level of the staining in the efferent endings was decreased from 74.28±11.71 to 46.5±7.78 (P<0.01).Conclusion In the cochlea of Mongolian gerbils, NOS staining was labeled in the auditory efferent endings at the base of OHCs,and ACh treatment could increase the NOS activity. These results indicate that ACh plays an important role by NO, a second messenger molecule, in the regulation of auditory physiology.
出处
《听力学及言语疾病杂志》
CAS
CSCD
2002年第3期156-158,T001,共4页
Journal of Audiology and Speech Pathology
基金
国家自然科学基金资助项目 (项目编号 39770 795 )
