摘要
目的 :研究人精子表面整合素亚基α5、β1在受精过程中的作用。方法 :以人精子穿透去透明带金黄地鼠卵异种体外受精试验 (SPA)作为检测人精子受精力的手段 ;分别用抗整合素亚基α5、β1抗体及整合素特异的配体RGD肽作用于精子后 ,观察其受精力的改变。结果 :用抗整合素亚基α5、β1抗体及RGD肽作用于精子后 ,受精率、受精指数及粘附指数均下降。结论 :人精子表面整合素亚基α5。
Objective: Using the in vitro sperm penetrating zona-free hamster eggs assay(SPA), we initially studied the effect of Integrins α 5?β 1 of human spermatozoa on fertilization. Methods: After being coincubated with anti-integrin α 5?β 1 antibodies and RGD tripeptide, sperms were used in SPA and the penetration rate, penetration index and adhesion index were been counted. Results: The penetration rate, penetration index and adhesion index of the test groups are lower than those of the two control groups. Conclusion: These results suggest that integrinα 5 ?β 1 play roles in sperm fertilization.
出处
《四川生理科学杂志》
2002年第1期20-22,共3页
Sichuan Journal of Physiological Sciences
关键词
整合素
人精子
受精
integrin
RGD
human sperms
fertilization
