摘要
目的 从诱导坏死和凋亡角度探讨丝裂霉素 (MMC)对膀胱癌EJ细胞的作用机制。 方法 将不同浓度的MMC作用于膀胱癌EJ细胞 ,在不同时间内采用流式细胞术 (FCM )和 3’末端脱氧核苷酸转移介导生物素 (TUNEL)及伊红排斥实验检测EJ细胞的凋亡情况和对其的杀伤作用。 结果 不同浓度的MMC在 2 4h内可以持续诱导EJ细胞凋亡 ,MMC 0 .1mg/ml组 2 4h时凋亡率为 71% ,对EJ细胞的杀伤作用与剂量呈正比。
Objective To present our attempt on investigate the effect of MMC on a human bladder cancer cell line, EJ cells. Methods EJ cells were maintained under tissue culture conditions in the presence of Mitomycin C (MMC), which had a potent cytotoxic effect on these cells. The apoptosis and necrosis of EJ cells were assessed and by using flow cytometry (FCM) ,TUNEL technology and eosin exclusion assay . Results Apoptosis of bladder cancer cell line EJ could be observed within the 24 h after the use of MMC in different doses.The apoptosis peak appeared in the 24th hour and a dose dependment effect could be observed. Conclusions MMC could inhibit the growth of bladder cancer cell via inducing apoptosis and necrosis of the cells.This might enclose the therapeutic mechanism of MMC on bladder cancer.
出处
《中华泌尿外科杂志》
CAS
CSCD
北大核心
2002年第6期368-369,共2页
Chinese Journal of Urology
基金
国家自然科学基金 (3 0 170 941)
