摘要
目的 探讨人玻璃体液对培养的人视网膜色素上皮 (retinal pigment epithelium,RPE)细胞和血管内皮细胞增生的影响。 方法 将人 RPE细胞和血管内皮细胞株 (vascular endothelial cell lines30 4 ,VEC30 4 )分别培养在 1∶ 8、1∶ 4、1∶ 2 (玻璃体液在总培养液中的体积比 )的人玻璃体条件培养液(vitreous- conditioned medium,VCM)中 ,其中 VCM分为有无血清 2组 ,在不同作用时间 (2～ 6 d) ,采用四唑盐 (tetrazolium,MTT)比色法检测 VCM对人 RPE细胞和 VEC30 4增生的影响。 结果 在有血清时 ,1∶ 4、1∶ 2的 VCM在培养的各时间段对 RPE细胞的促增生作用与对照组比较 ,差异有非常显著性意义(P<0 .0 1) ,对 VEC30 4的抑增生作用与对照组比较 ,差异有显著性意义 (P<0 .0 5 ) ;在无血清时 ,1∶ 2的VCM在培养 2 d时对 RPE细胞的促增生作用与对照组比较 ,差异有显著性意义 (P<0 .0 5 ) ,在培养 4、6 d时的差异有非常显著性意义 (P<0 .0 1)。 结论 一定体积分数的人 VCM促进人 RPE细胞的增生 ,抑制VEC30 4的增生 ,提示玻璃体液在增生性玻璃体视网膜病变 (proliferative vitreoretinopathy,PVR)和眼内血管增生性疾病中可能发挥不同的调控作用。
Purpose To investigate the effects of human vitreous fluid on proliferation of cultured human retinal pigment epithelial (RPE) cells and vascular endothelial cell lines(VEC304). Methods Human RPE cells and VEC304 were cultured and treated in different human vitreous conditioned medium (VCM) with or without serum, vitreous volume concentrations of VCM were 1∶8, 1∶4 and 1∶2. Cells proliferation was assayed by tetrazolium (MTT) colorimetry at the 2nd, 4th and 6th day respectively. Results In the presence of serum, 1∶4, 1∶2 VCM had a significantly stimulative effect on RPE cells proliferation compared with control group at the 2nd, 4th, and 6th day retrospectively ( P< 0 01), but exerted a strongly inhibitory effect on VEC304 proliferation compared with control group at the 2nd, 4th, and 6th day retrospectively ( P <0 05). In the absence of serum, only 1∶2 VCM had a stimulative effect on RPE cells growth compared with control group at the 2nd day ( P< 0.05) and obviously at the 4th and 6th day respectively ( P< 0 01). Conclusion Human vitreous fluid influences human RPE cells and VEC304 growth in vitro. This result suggests that vitreous may play different role in proliferative vitreoretinopathy and intraocular neovascular disease.
出处
《中华眼底病杂志》
CAS
CSCD
2002年第2期140-142,共3页
Chinese Journal of Ocular Fundus Diseases
