摘要
运用组织培养技术,通过孢子繁殖、无性无融合生殖和愈伤组织分化不定芽等方式得到桫椤(Alsophila spinulosa)幼苗。成熟的新鲜的桫椤孢子有近1年的休眠期。用50ppmGA处理孢子2—5分钟,是打破孢子休眠的较好措施。新鲜孢子在室温下寿命约8天;在10℃下贮存,孢子寿命达1年以上;而经适当干燥处理后于10℃下贮存的孢子,寿命达450天。在实验室内,控制培养条件可保存桫椤的丝状体、原叶体和孢子体。这对于不适宜低温贮藏的热带植物的保存是一种经济、有效的方法。
Sporophytes of Alsophila spinulosa were obtained from spore propagation agamospory and adventitious bud produced from callus by tissue culture. The morphology of prothallus is plastic. In the tissue culture, the prothallus had three forms, normal long cordate prothallium, abnormal ligulatc prothallium and cylindrical prothallium, and then they could produce normal plant. When BM was a improved MS, the spore germination, filamentous growth and transition from one to two-dimensional growth, prothallium development and sporophyte differentiation etc. were influenced by sugar concentration, inorganic salts concentration, composition and proportion of hormone. The sugar concentration by 0-1 per cent is suited for spore germination, but it helps differentiated sporophyte by 3-5 per cent. The days of differentiation sporophyte are shortening(f rom 96 to 48 days) and the freqency of differentiation plantlet is raising (from 25.8 to 46.2%) along with the reduction of inorganie salts concentration (from 1/5 to 1/10). The sporophyte is gctting better indueed as improved MS + KT lmg/1 + 2, 4-D 0.4mg/l. Adult and fresh spores of A. spinulosa had a dormant period for one year. The dormancy was broken, when- the speres were submerged in solution containing 50 ppm GA for 2-5 minules. In a room (24±5℃), the viability of spore had only 7- 8 days; in cold storage condition (10℃), the life span of spore would continue over a year; in the condition of under lower (10℃) and suitable desiccation, the life of spore would attain about 450 days.
Filamentous prothallus and sporophytes of A. spinulosa can be storaged in the laboratory (24±5℃) with controlcd culture condition by tissue culture. Large numbers of tropical plant can not survive for long term in low temperature so the tissue culture method is possible to conserve tropical germplasm in room temperature. It is also economic and applicable to wide range of species.
出处
《云南植物研究》
CSCD
1991年第2期181-188,共8页
Acta Botanica Yunnanica
基金
林业部"七.五"重点攻关项目基金资助
关键词
桫椤
组织培养
繁殖
种质保存
Alsophila spinulosa
Tissue culture
Way of propagation
Conservation of germplasm
