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溶血样品胆碱酯酶活力实验研究

Experimental Study on the Activity of Cholinesterase of Hemolyzed Blood Samples
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摘要 目的 探讨溶血样品胆碱酯酶(h-ChE)与未溶血样品胆碱酯酶(bl-ChE)活力值之间的关系。方法 采用羟肟酸铁比色法测定济南地区112名健康人bl-ChE及h-ChE,并用血红蛋白(Hb)值对h-ChE进行校正。结果 bl-ChE及h-ChE活力值之间呈高度正相关(r=0.728,P <0.01)。结论 Hb值校正h-ChE可避免由样品溶血干扰比色所造成的测定值误差,缩小男、女人群之间的测定值离散度,提高了胆碱酯酶测定值的可信性。 Objective To explore the relationship between the activity of cholinesterase in hemolyzed blood sample and non-hemolyzed blood sample. Methods The activities of cholinesterase in hemolyzed blood samples and non-hernolyzed blood samples from 112 healthy individuals in Ji'nan were determined by the ferric trichloride chro-mometry, and the activities of cholinesterase in hemolyzed blood samples were adjusted by the concentration of ho-moglobulin (Hb) in blood samples. Results Highly significant correlation was found in the activities of cholinesterase between hemolyzed blood samples and non-hemolyzed blood samples (r = 0.728, F<0.01) . Conclusion The adjustment of the activities of cholinesterase by concentration of Hb in hemolyzed blood samples could minimized the measurement errors induced by the interference of hemolysis in the process of chromometry, and reduced the dispersion of data on the activities of cholmesterase between male and female, and also could improve the reliability of the data obtained.
出处 《环境与健康杂志》 CAS CSCD 北大核心 2002年第4期336-337,共2页 Journal of Environment and Health
关键词 溶血样品 实验研究 健康人 胆碱酯酶 活力 血红蛋白 有机磷农药 Healthy person Cholinesterase Activity Hemoglobulin
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