摘要
In order to investigafe the changes of cireumstance of fertilization at the time pre-andpost-ovulation,the amino acid cantent of cervix mucus in 27 cows were determined separately.Theresults indicated that the amino acid content at the time pre-orulatlon in arerage was 1032 pg/mL,and2380 ug/mL at the time post ovulation which made up 2.3 times of the former.The highest proportions of determined amino acids in cervix mucus were those af glutamic acid andthreonine making up 12.8%and 12.2%of the total,respectively,and the lowest was thaf of histidine,making up only 2.2%of the total.Those proportions stand stable at the time pre-and post-ovulation.The threonine content in cervix mucus of the tested sample cows at the time post-ovulation dropped onan average of 19%of that at pre-ovulation.And,Iysine increased at post-ovulation on an average25%of that at pre-ovulation.The amino acid metabolism in body fluid is an important physiological activity,in cervix mucus afteroyulation the bath cases of dropping of threanine content and the enhancement of Iysine reflect thechanges in protein composition which were related to those amino acids.As for the aforesaid changes.it stil needs further research on the relating physiological significance and the producing influenceupon the combinatioa between egg and X er Y sperm.
为了研究母体排卵前后受精环境的变化,测定了27头奶牛排卵前和排卵后宫颈粘液中的氨基酸含量,测定结果表明,宫颈粘液的氨基酸含量排卵前平均为1032μg/mL,排卵后平均为2380μg/mL,排卵后为排卵前的2.3倍(Table 1)。排卵前,宫颈粘液中含量比例最高的氨基酸是谷氨酸和苏氨酸,分别占氨基酸总量的12.8%和12.2%,含量比例最低的最组氨酸,只占2.2%,这种比例在排卵前后都是比较稳定的(Table2)。排卵后,宫颈粘液中氨基酸比较普遍下降的是苏氨酸,比排卵前平均下降19%,排卵后普遍增加的是赖氨酸,比排卵前平均增加25%(Table 3)。Fig.1表明:排卵前后,宫颈粘液中均不含游离氨基酸。体液中的氨基酸代谢是一种重要的生理活动,排卵后宫颈粘液中苏氨酸含量普遍降低,赖氨酸含量普遍增加的生理活动反映了这些氨基酸相关的蛋白成分发生了变化,这些蛋白成份的变化有否生理意义,是否对卵与X或Y精子的结合产生影响,有待深入研究。
