摘要
Objective:To understandtheclinicalsignificanceof CD4 + naturalkillerT(NK-T)cellsin chronic hepatitisB virus(HBV)infection.Methods :Peripheralbloodmononuclearcells(PBMCs)fromindividualswith chronicHBVinfectionwereseparated routinely.AfterInductionwithIL-12/IL-2for12d,theproportionof CD4 + NK-Tcellsin peripheralbloodwas determinedby fluorescenceactivatedcellsorter(FACS)analysis,andthe cytotoxicityof peripheralbloodlymphocytes(PBLs)was testedwitha4 h 51 Cr releaseassay.Results:After IL-12/IL-2induction,theproportionof CD4 + NK-Tcellswas(18.1±4.20)%, (6.95±2.85)%and(1.50±1.30)%inthe healthycontrol,CAHandAsCrespectively.Thatintheperipheralbloodof chronicHBVinfectedindividualswas lowerthanthatin thehealthycontrol.CD8+NK-Tcellswas(2.70±1.10)%, (2.20±1.40)%and(3.10±0.70)%respectively.In vitro cytotoxicityassaysagainstWishcellsrevealedthatthePBLscytotoxicityreducedin chronic HBVinfectedindividuals(P<0.05),andthatin AsCgroupwas significantlylowerin comparisonwithCHBand healthycontrolgroups.Thecytotoxicityof CD4 + NK-TcellsagainstWishcellscouldbe abolishedby treatingPBLs witheitheranti-CD4Abor anti-CD56Ab andcomplement,andpartiallydepleted by anti-CD8Ab.Conclusion:Theabnormalcellularimmunefunctionof chronicHBVinfectedindividualsmaybe associatedwiththedeficiency of CD4 + NK-Tcells.
Objective:To understandtheclinicalsignificanceof CD4 + naturalkillerT(NK-T)cellsin chronic hepatitisB virus(HBV)infection.Methods :Peripheralbloodmononuclearcells(PBMCs)fromindividualswith chronicHBVinfectionwereseparated routinely.AfterInductionwithIL-12/IL-2for12d,theproportionof CD4 + NK-Tcellsin peripheralbloodwas determinedby fluorescenceactivatedcellsorter(FACS)analysis,andthe cytotoxicityof peripheralbloodlymphocytes(PBLs)was testedwitha4 h 51 Cr releaseassay.Results:After IL-12/IL-2induction,theproportionof CD4 + NK-Tcellswas(18.1±4.20)%, (6.95±2.85)%and(1.50±1.30)%inthe healthycontrol,CAHandAsCrespectively.Thatintheperipheralbloodof chronicHBVinfectedindividualswas lowerthanthatin thehealthycontrol.CD8+NK-Tcellswas(2.70±1.10)%, (2.20±1.40)%and(3.10±0.70)%respectively.In vitro cytotoxicityassaysagainstWishcellsrevealedthatthePBLscytotoxicityreducedin chronic HBVinfectedindividuals(P<0.05),andthatin AsCgroupwas significantlylowerin comparisonwithCHBand healthycontrolgroups.Thecytotoxicityof CD4 + NK-TcellsagainstWishcellscouldbe abolishedby treatingPBLs witheitheranti-CD4Abor anti-CD56Ab andcomplement,andpartiallydepleted by anti-CD8Ab.Conclusion:Theabnormalcellularimmunefunctionof chronicHBVinfectedindividualsmaybe associatedwiththedeficiency of CD4 + NK-Tcells.
