摘要
运用小规模实验初步探讨了以中国人群为基础的遗传图绘制工作的必要性。 18个无关汉族 3代家系共 131份血样采自甘肃省白银地区 ,常规PCR扩增 9号染色体的 10个STR基因座 ,采用非变性聚丙烯酰胺凝胶电泳分析。PCR产物经克隆测序确定核心序列重复次数 ,采用标准命名法命名各等位基因 ,用POPGENE软件包计算各基因座等位基因频率 ,并进行Hardy Weiberg平衡检验 ,用Linkage软件包进行各基因座之间连锁关系分析。根据连锁分析结果绘制了中国人群由 10个STR基因座构成的 9号染色体遗传图。基于中国人群体的 9号染色体 10个STR连锁分析结果与GDB检索结果之间存在较显著的差异 ,这种差异同时表现在个别基因座之间和 9号染色体遗传图总长度上。男、女遗传图总长度分别为 12 9.2 1cM和 178.4cM ,均大于高加索人。说明了在运用GDB数据之前 ,有必要根据实验群体进行基因座的初步评估 。
To understand the genetic polymorphism of 10 STR loci on chromosome 9 and reveal the difference on the linkage maps of different populations, the author used a set of nine tetranucleotide ?and one tritranucleotide repeat STR markers on chromosome 9 chosen from Genome Data Bank. EDTA blood was collected from unrelated family in Baiyin City, Gansu province. DNA was extracted by Chelex 100 method and amplified by the polymerase chain reaction (PCR). The PCR products were analyzed using PAGE electrophoresis. We calculated the allele frequencies and heterozygosity and PIC of every ten STR loci. Using the data of Chinese Han pedigree, we constructed a genetic map of chromosome 9 making up with 10 STR loci. Then we compared our map with the map in GDB. Our results indicate that there are some differences on the genetic map between Chinese and GDB. That means, it is essential to do some population genetic evaluations before using the data of GDB into Chinese population.
基金
CMB(96-63 6)
