摘要
红豆草下胚轴切段在含有1mg/1BA,1mg/1KT的LS培养基上培养,两周后在切面处产生愈伤组织.选择由一块切段产生的淡黄色愈伤组织,繁殖并连续选择几代至其外观稳定,转移到含1mg/1BA的LS培养基上,15天后即可观察到有大量的球形胚形成,但只有少数可按正常的胚胎发育途径发育.其存在的另一可能途径是球芽途径,即球状结构上逐渐分化出根和芽而得到完整植株,它具有独立的结构,不同于一般的器官发生途径.
Hypocotylar explants of Onobrychis viciaefolia Scop were cultured on LS basal medium-supplemented with 1 nig/1 BA, lmg/1 KT. Two weeks after of culture calli were initiated on the surface of sections. Light-yellow callus from one explants was selected and proliferated on LS medium. And then it transferrated to somatic embryo induction medium (LS basal medium with lmg/1 BA). Contact embryogenic calli containing a large number of globular embryos were found after 15 days of embryogenic culture, but only a few globular embryos can develop normally. Another new possible pathway for in vitro development is. buds and roots were formed on the surface of globular-like structure and it is different from normal organogenesis because of its individual structure.
出处
《华北农学报》
CSCD
北大核心
1989年第3期11-15,共5页
Acta Agriculturae Boreali-Sinica
关键词
红豆草
组织培养
体细胞胚
克隆
Sainfoin
Tissue culture
Somatic embryo
Cloning
