摘要
本文采用营养缺陷型的互补进行了啤酒酵母原生质体融合产物的筛选。从DNA含量分析看,融合子多为三倍体,少数为四倍体,融合子的生长速度和酒精产率都比亲株高,种内原生质体融合率为1.21×10^(-4)。
Prototrophic hybrids have been obtained by the protoplast fusion between auxotrophic haploid and diploid mutants of Saccharomyces cerevisiae. Nutritionally-complemented stable heterozygous triploid and tetraploid hybrids were confirmed by cell DNA content. The growth rate and fermentation rate of hybrids were much faster, and productivity of alcohol was 9.1-22.7% higher than that of the prototrophic parental cell strain. The frequency of protoplast fusion was 1.21×10-4.
出处
《云南大学学报（自然科学版）》
CAS
CSCD
1991年第1期70-74,共5页
Journal of Yunnan University(Natural Sciences Edition)
