摘要
经过赤霉素、3-吲哚乙酸、6-苄氨基嘌呤和层积三种组合处理的玉兰种子表现出不同的休眠解除速度。研究了这三种处理过程中种子脂肪、RNA和可溶性蛋白质的变化,结果表明,脂肪和56.7KD蛋白质含量的减少与休眠解除呈正相关。在休眠解除过程中,RNA、18.3KD蛋白质和总可溶性蛋白质含量都先增加后下降,同时还有39.7KD的新蛋白质谱带出现。蛋白质合成的开始期比RNA合成提前 3周。因此,在玉兰种子休眠解除过程中,有贮藏物(脂肪和蛋白质)的动员、蛋白质和RNA的合成;玉兰种子休眠解除在早期受到翻译水平的控制,后来又受到转录水平的调节。
M.denudata seeds were treated by three combinations o?gibberellic acid + 6 - benzylamiaopurine + indolylacetic acid + stratification, and significant differences were found for the rate of dormancy breakagy (P<0.05). Study of the changes in seed fat, RNA and protein contents during the three treatments showed that decreases in the contents of fat and 56.7 KD protein were positively related to dormancy release. During removal of dormancy, the contents of RNA, 18.3KD protein and total soluble protein increased gradually followed by declines at the late stage, while a new protein ( on electrophoretic gel ) was pro-duced.Protein synthesis was initiated 3 weeks earlier than RNA synthe-sis. It is suggested that the metabolic requirements for removal of M. denudata seed dormancy involve reserve ( fat and protein ) mobilization, as well as protein and RNA synthesis, and that dormancy breakage is controlled at the level of translation at the initial stage, and then the regulation at the level of transcription is involved.
出处
《园艺学报》
CAS
CSCD
北大核心
1991年第1期75-80,共6页
Acta Horticulturae Sinica
关键词
玉兰
休眠
核酸
蛋白
贮藏物
Seed dormancy
Magnolia denudata
RNA
Protein
Reserve mobilization
