摘要
目的 研究总丹酚酸对脑缺血再灌注损伤的保护作用。方法 采用跳台法和断头法 ,观察总丹酚酸对缺血再灌注小鼠学习记忆功能障碍的作用和耐缺氧能力的影响。采用化学法 ,观察总丹酚酸对缺血再灌注小鼠脑组织中超氧化物歧化酶的 (SOD)活性 ,丙二醛 (MDA)和谷胱甘肽过氧化物酶 (GSH)的含量。结果 总丹酚酸改善缺血再灌注引起的学习记忆障碍 ,缩短被动回避反应时间 ,减少错误次数 ,延长潜伏期 ,延长断头后呼吸持续时间。总丹酚酸亦增强缺血再灌注小鼠脑组织SOD的活性 ,降低MDA含量 ,增加GSH的含量。结论 总丹酚酸对脑缺血再灌注损伤有显著的保护作用 。
AIM To study the protective effect of total salvianolic acid against cerebral ischemia reperfusion injury. METHODS The cerebral ischemia reperfusion model in mice was made by means of ligating bilateral common carotid arteries in mice. After reperfusion, latency, error number of step down test and the gasping time after cutting head in ischemia reperfusion mice were recorded. Spectrophotometric assay were used to measure the activity of superoxide dismutase (SOD), the contents of malondialdehyde (MDA) and glutathione peroxidase (GSH) in brain of experimental mice brain homogenate. RESULTS In step down test, the ischemia reperfusion impaired the function of learning and memory in mice. The total salvianolic acid markedly improved the function of learning and memory, reduced the error number and extended the latency in ischemia reperfusion mice. The total salvianolic acid also significantly inhibited the changes of SOD, MDA and GSH in the cerebrum induced by ischemia reperfusion. CONCLUSION The total salvianolic acid has protective effects against cerebral ischemia reperfusion injury via its antioxidant activity.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2002年第3期275-277,共3页
Chinese Pharmacological Bulletin
基金
国家科委"10 3 5"项目
新药基金资助课题 NoK95 12 2 7
关键词
总丹酚酸
脑缺血再灌注
抗氧化
total salvianolic acid
ischemia-reperfusion
antioxidant activity
