摘要
通过摇瓶与土柱试验对硅酸盐细菌NBT菌株的释钾条件进行了研究。摇瓶试验表明 ,pH值、装液量、土壤矿物种类、菌株特性均对硅酸盐细菌的释钾效能有重要影响。pH 6 .5～ 8.0时NBT菌株的释钾效能最高 ,接活菌比接灭活菌对照溶液中的钾含量增加 84 .8%～ 12 7.9%。在 2 5 0ml三角瓶中装液量为 4 0ml,接菌处理溶液中的钾比接灭活菌对照增加 12 6 .3% ,而装液量为 10 0ml,接菌处理溶液中的钾比对照仅增加 87.2 % ;硅酸盐细菌NBT菌株对供试矿物的分解能力为伊利石 >钾长石 >白云母。在供试的不同菌株中 ,硅酸盐细菌NBT菌株的释钾能力最强 ,2 8℃振荡培养 7d ,NBT菌株释放出的钾达 35 .2mg/L ,比其它供试菌株释放出的钾增加 31.8%～ 12 0 3.7%。土柱试验表明 ,硅酸盐细菌NBT菌株在 2种供试土壤中能够存活并表现出一定的解钾作用。接菌处理土壤中硅酸盐细菌细胞数量由 2 .6～ 3.0× 10 6个 /g土增加到 6 .8～ 7.4× 10 7个 /g土。NBT菌株在黄棕壤和水稻土中 2 8℃培养 7d后 ,土壤中的速效钾分别增加 31.2～ 33.6mg/kg土和 2 1.7mg/kg土 ,分别比接灭活菌对照增加 2 90 .6 %和 185 .5 %。方差分析表明 ,差异达显著水平。
Study on the conditions of potassium release from the minerals was carried out through shake flask study and soil column experiment. The results showed that potassium release from the minerals was obviously affected by pH, DO, types of soils and minerals, and the strain used. The strain NBT had the potential of releasing potassium under the suitable pH (pH 6.5-8.0). The content of potassium in solution inoculated with bacteria was increased by 84.8%-127.9% compared with the control inoculated with dead bacteria. Under the 40ml media in 250ml Elenmeyer flaskon, the content of potassium released by the cells was increased by 39.1% compared with that of potassium released under the 100ml media. The effect of potassium bearing minerals decomposed was as follows: illite>feldspar>muscovite. The strain NBT had the maximum efficacy of releasing potassium in the strains tested. In agitated flask, at 28℃ for 7 days, the strain NBT had the potential of releasing 35.2 mg/L, increased by 31.8%-1203.7% compared with other strains. The growth and action of strain NBT was also affected by the soils. The soil column test showed that the cells in the soils could produce from 2.6 -3.0×10 6/g soil to 6.8-7.4×10 7/g soil. The available potassium was increased by 31.2-33.6mg/kg soil (yellow brown soil )and 21.7mg/kg soil (paddy soil) by the strain NBT, increased by 290.6%(yellow brown soil ) and 185.5%(paddy soil). Biostatistical analysis showed that the variance was obvious.
出处
《中国农业科学》
CAS
CSCD
北大核心
2002年第6期673-677,共5页
Scientia Agricultura Sinica
