摘要
以冷冻丙酮制备莲藕多酚氧化酶丙酮粉 ,用分光光度法研究pH值、温度、底物浓度、抑制剂对酶活性的影响 .结果表明 ,以邻苯二酚为底物 ,莲藕ppo的最适pH值为 7.0 ,最适温度为 35℃ ;Km为 2 2 .7mmol/L ,Vm为 0 .6 2 5OD/min ,10 0℃热处理 2 5s可钝化ppo的活性 ;抗坏血酸、亚硫酸钠为强烈抑制剂 ,1.75× 10 -4的抗坏血酸和 3.0 0× 10 -4的亚硫酸钠能有效抑制ppo的活性 .
The acetone powder of ppo in lotus root being extracted from ice?cold acetone,the effects of pH,temperature,concentration of substrate,and inhibitors on ppo's activity were investigated by using the spectrophotometric method.The results showed that the optimal pH and tempearture were 7.0 and 35 ℃,Km and Vm of ppo with catechol substrate were 22.7 mmol/L and 0.625 OD/min respectively,the extinct condition of ppo was 25 s at 100 ℃,sodium sulfite and ascorbic acid were strong inhibitors,with the effective concentration being 3.00×10 -4 and 1.75×10 -4 respectively.
出处
《吉首大学学报（自然科学版）》
CAS
2002年第2期82-84,共3页
Journal of Jishou University(Natural Sciences Edition)
