摘要
以甲壳素的浓盐酸水解产物氨基葡萄糖盐酸盐为原料 ,通过阴离子交换层析法制备氨基葡萄糖硫酸盐 ,并对其的部分性质进行研究。采用本方法制备的氨基葡萄糖硫酸盐产物的氨基葡萄糖含量为 71.6 0 % ,硫酸根含量为 18.2 5 % ,氯离子含量为 0 .0 38%。经纸层析鉴定 ,以吡啶、乙酸乙酯、水和冰醋酸按体积比 5∶5∶3∶1组成的溶剂系统进行展层 ,氨基葡萄糖硫酸盐的Rf 值为 0 .36。将氨基葡萄糖硫酸盐产物的晶体重新溶解于 2 0℃水中时 ,它的比旋光度随时间的延长而下降 ,1.5h后趋于稳定 ,从 92 .1°降低至 5 6 .7°。对它的红外光谱鉴定表明 ,在波数为 330 3.9、14 15 .6、12 15 .6、1181.2、1118.7、10 93.7和 10 34.4cm-1处有特征吸收峰。通过对氨基葡萄糖硫酸盐产物的元素分析 ,碳、氢、氮和硫的含量分别为 2 9.6 3%、6 .34%、5 .39%和 6 .5 6 %。
By using anion exchange column chromatography, glucosamine sulfate was prepared from glucosamine hydrochloride that was produced by acidic hydrolysis of chitin and some properties of the product were studied. The contents of glucosamine, sulfate radical and chloridion in the product were 71.60%, 18.25% and 0.038%, respectively. The R f value of the glucosamine sulfate was 0.36 measured by paper chromatography with the solvent system of pyridine, ethyl acetate, water and glacial acetic acid (5∶5∶3∶1 in volume). While redissolved in water at 20℃, the specific rotation of glucosamine sulfate decreased with the time from 92.1° to about 56.7° and was, however, stable after 1.5 h at 56.7°. It exhibited the characteristic absorption bands at 3307.9, 1415.6, 1215.6, 1181.2, 1118.7, 1093.7 and 1034.4 cm -1 in IR spectrum. The elemental analysis for the glucosamine sulfate showed that the contents of carbon, hydrogen, nitrogen and sulfur were 29.63, 6.34, 5.39 and 6.56 %, respectively.
出处
《上海水产大学学报》
CSCD
2002年第2期145-148,共4页
Journal of Shanghai Fisheries University
关键词
氨基葡萄糖硫酸盐
离子交换层析
纸层析
红外光谱
元素分析
glucosamine sulfate
an ion exchange chromatography
paper chromatography
infrared spectrum
elemental analysis
