摘要
目的 观察异紫堇啡碱 (ISOC)对去甲肾上腺素 (NA)和高钾所致培养乳鼠心肌细胞内游离钙浓度([Ca2 + ]i)增高的影响 ,初步分析该药心脏效应的作用原理。方法 利用钙荧光指示剂Fura - 2 /AM负载的培养乳鼠心肌细胞 ,动态地观察在ISOC存在下 ,由NA和高钾所增加的 [Ca2 + ]i 改变。结果 ISOC对培养乳鼠心肌细胞的静息 [Ca2 + ]i 无影响 ,其在 10 -5和 3× 10 -5mol/L时也不显著抑制高钾所致心肌细胞 [Ca2 + ]i 的增高 ,但能降低由NA所升高的心肌细胞 [Ca2 + ]i。结论 ISOC抑制由受体中介的心肌细胞 [Ca2 + ]i
Objective To analyse the antiarrhythmic mechanism(s) of isocorydine(ISOC),its effects on the increasing of the cytosolic free Ca 2+ level ([Ca 2+ ] i) induced by noradrenaline (NA) and high potassium in the cultural newborn rat beart cells were investigated.Methods The changes of the [Ca 2+ ] i increased by NA and high potassium in the presence of ISOC were determined, using fura-2/AM laoded cultural beart cells of the newborn rat.Results ISOC at 10 -5 and 3×10 -5 mol /L -1 had no effect on the basic [Ca 2+ ] i and the [Ca 2+ ] i increased by high potassium, bnt it inbibited the [Ca 2+ ] i increased by NA significantly.Conclusions ISOC inhibited the receptor mediated increasing of [Ca 2+ ] i in the heart cells,and it may be partly relate to its antiarrhythmic effects.
出处
《合肥医学院学报》
2002年第2期97-99,共3页
Journal of Zunyi Medical University
基金
贵州省科技厅基金 (黔计字 94 30 33)
关键词
异紫堇啡碱
乳鼠
心肌细胞
细胞内游离钙
血管扩张药
Isocorydine
newborn rat beart cell
noradrenaline
high potassium
cytosolic free [Ca 2+ ] i
