摘要
目的:探讨婴幼儿血管瘤增生及凋亡的过程,为临床治疗提供参考。方法:选取术前未经治疗的38例血管瘤手术切除标本,采用免疫组化增殖细胞核抗原（PCNA）及末端脱氧核苷酸转移酶介导的脱氧尿苷三磷酸切口末端标记法（TUNEL）,检测其PCNA指数及凋亡指数（AI）。结果:增生期血管瘤婴幼儿的平均年龄为（4.6±3.0）个月,PCNA指数为3.27±0.60,AI为1.69±0.96;退化期血管瘤婴幼儿的平均年龄为（17.0±7.2）个月,PCNA指数为1.39±0.50,AI为26±13.93,两组差异有显著性。结论:血管内皮细胞增生及凋亡存在于婴幼儿血管瘤的整个自然过程中:在增生期,其血管内皮细胞的增殖活性大于凋亡的活性,血管瘤呈增大趋势;在退化期,其血管内皮细胞的凋亡活性大于增殖的活性,血管瘤呈缩小趋势。
Objective: To explore the proliferation and apoptosis of infantile hemangioma. Methods:Samples of hemangioma resected from 38 cases non-treated pre-operatively were examined and the PCNA index and apoptosis index were detected by the method of Immuno-histochemical PCNA and TUNEL. Results: In the proliferative phase of hemangioma in infants with mean age(4.60±3.04)m, the PCNA index was 3.27±0.60 and AI was 1.69±0.96; In the phase of hemangiomas in infants with mean age(17.04±7.23)m, the PCNA index was 1.39±0.50 and AI was 26±13.39. There was significant difference between the two groups. Conclusions: The proliferation and apoptosis of endothelial cell was always present in whole natural process of infantile hemangioma. In the proliferative phase, the activation of proliferation was higher than the apoptosis and the hemangioma had a tendancy towards growth; while in the retrogressive phase, the apoptosis was more significant, so the hemangioma became shrinkage.
出处
《诊断学理论与实践》
2002年第2期90-91,96,共3页
Journal of Diagnostics Concepts & Practice
