摘要
以小麦根腐病菌002号菌株产生的致病培养滤液为选择剂,选用春、冬小麦品种、品系以及杂种的花药和幼德进行离体培养,并结合物理诱变技术,已获得抗根腐病的植株,用根腐病菌分生孢子接种鉴定,再生植株50株中有12株抗病。
The fungal filtrate prepared from the cultures of H. sativum train No. 002 was used as screening agent. The anther and young inflorecences from varieties, strains and hybrids of spring and winter wheat were in vitro cultured. The calli were treated with physical mutagens. Finally the mutants resisting to H. sativum were obtained. Out of 50 regenerated plants resisting to fungal filtrate, 12 plants were resistant when put them to inoculation test with conisiospore of H. sativum.
基金
国家自然科学基金
关键词
小麦
根腐病
抗病突变体
筛选
离体
Helminlhosporium sativum in wheat, Resistant mutant, In vitro selection
