摘要
为了确定胞质分裂阻滞微核测试法(CB-MNT)的实用价值,本研究用0.025-0.4μg/mL的丝裂霉素C(MMC)处理培养的人体外周血淋巴细胞,比较CB-与常规(C)-MNT检测遗传毒性的敏感性,结果表明:(1)在整个剂量范围内,两法测得的微核率(MNF)均有良好的剂量效应关系,但CB法测得MNF低于常规法,随剂量增加而加著;CB法测知的最低浓度为0.1μg/ml,常规法为0.025μg/ml,可见常规法的敏感性优于CB法;(2)我们首次比较了CB法与常规法所检测微核的平均体积,发现CB法检测微核的体积接近常规法的3倍,并提出由于微核形成前后,微核间及微核与主核间的融合,导致了微核体积的增大和数量的减少,进而引起了MNF的下降;(3)CB法检测健康人自发MNF明显高于常规法,这可能是CB法检测低剂量诱变剂效应,较常规法不够敏感的重要原因之一。最后作者结合文献讨论了CB-与C-MNT的实用价值。
The authers have studied gf notoxicological effects of MMC by means of CB- and C-MNT to evaluate practical value of CB-MNT. The main results are as follows: (1) In 0.025-0.4 μg/ml of MMC MNFs in lymphocytes detected by CB- and C-MNT presented dosedependent increase, but the MNT detected by C-MNT was higher than that by CB-MNT, this difference became greater as MMC concentrations were increased. The minimum concentrations were 0.1 μg/ml for CB-MNT and 0.025μg/ml for C-MNT respectively. There fore C-MNT is more sensitive to MMC than to CB-MNT; (2) The mean volume of MN detected by CB- and C-MNT was estimated separately. The volume of MN detected by CB-MNT was about 3 times as large as that by C-MNT. The authers suggested that the fusion between micronuclei in cytoplasm of CB- cells could lead to increase of MN volume and decease of MN numbers; (3) In healthy donors the background MNP in lymphocytes detected by CB-MNT was significantly higher than that by C-MNT, it could be one of the reasons that led to the decrease of the sensitivity of CB-MNT to low doses of mutagens. Finally, the practical value of CB- and C-MNT was discussed.
关键词
淋巴细胞
微核测试法
细胞分裂阻滞
The micronucleus rest, Cytokinesis-block conventional method, Human lymphocyte, Mitomyein C
