摘要
在附加激素的MS培养基上,培养党参下胚轴和无菌芽切段,诱导产生愈伤组织并且再生植株。经过两年多(15个世代)的继代培养,建立了党参体细胞无性系。实验结果表明:(1)培养基MS+0.4mg/L 2,4-D+0.8mg/L Kt+2.0mg/L IAA对愈伤组织诱导及继代培养,MS+0.2mg/L 6-BA诱导外植体产生丛芽和愈伤组织再分化,MS+0.5mg/L NAA+0.2mg/L 6-BA及MS+0.2mg/L NAA诱导生根效果最好。(2)愈伤组织再分化经过胚状体途径。
MS medium supplemented with some phytohormones were used for the organ and tissue culture of Dangshen (Codonopsis filosula Nannf). Callus induction and plantlet regeneration were achieved from hypocotyl and stem segments of Dangshen. Somatic clones were established after 15 generations of subculture for more than two years. We obtained in our experiments the following results: (1) MS + 0.4mg/L 2.4-D + 2.0 mg/L lAA + 0.8mg/L Kt for callus induction and subculture, MS+ 0.2 mg/L 6-BA for induction of clustered buds and plant-let regeneration, and MS+ 0.5 mg/L NAA +0.2 mg/L 6-BA and MS+ 0.2 mg/L NAA for root induction were found to be the most suitable. (2) The redifferentiation of calli was via embryoid pathway.
关键词
党参
离体培养
植株再生
Phytohormoncs, Somatic clones, Clustered buds, Subculture
