摘要
目的 研究视网膜锥体细胞功能异常大鼠的视觉电生理特点 .方法 按国际临床视觉电生理学会标准化方案 ,采用 RETIport系统和自制的氯化银角膜电极、不锈钢针状电极对成年雄性 SD大鼠观察视网膜电图暗适应视网膜电图(ERG)、振荡电位 (OPs波 )和明适应 ERG和颜色光 ERG;采用 RETIscan系统观察明暗适应条件下的白色和颜色光多焦点视网膜电图 (m ERG) .颜色光 ERG和 m ERG记录通过在被测眼前分别加红、绿、蓝色滤光片后进行 .结果 大鼠 ERG和 OPs波形与人类相似 .正常大鼠的白色和颜色光 m ERG均能够记录到 .在暗适应条件下 ,m ERG总和反应 (n=5大鼠 )的 b波幅值和潜伏期分别为 :(12 6± 4 1)μV,(44 .8± 2 .4 )ms(白 ) ;(10 4± 39) μV,(47.8± 0 .8) ms(绿 ) ;(86± 33)μV,(47.2± 1.9) ms (蓝 )和 (76± 2 1)μV,(6 5 .0± 1.9) m s(红 ) .明适应条件下正常大鼠的 m ERG仍能够记录到 ,但是波幅值较低 .电生理异常大鼠 ERG和 OPs波形未见异常 ,明适应 ERG和 m ERG波形消失 .暗适应条件下的白光 m ERG波形难以辨别 ,但是颜色光 m ERG波形可以记录到 ,其总和反应 b波幅值和潜伏期 (n=2只眼 )分别为 :(42± 7) μV,(5 0± 7) ms (绿 ) ;(31.1± 0 .4 )μV,(5 0± 8) ms (蓝 ) ;(12 5±4 1) μV,(5 7±
AIM To investigate the visual electrophysiological properties of a SD rat with retinal cone cell degeneration. METHODS According to the International Clinic Visual Electrophysiological Standard Program, five commonly obtained responses (scotopic and photopic electroretinograms and oscillatory potentials) and chromatic stimulus ERG were recorded with RETIport system. Achromatic and chromatic stimuli multifocal retinoelectrograms (mERG) were recorded with RETIport system in dark and light adaptations, respectively. The chromatic ERG and mERG were recorded by putting a color filter (Green, Blue and Red) before the testing eyes. RESULTS ERGs and OPs waves of the normal rats were similar to those of human. The achromatic and chromatic stimuli multifocal retinoelectrograms could also be recorded in normal rats. In the dark adaptation, the amplitude and latency of b waves (sum response) of achromatic and chromatic mERG in normal rats ( n =5) were (126±41) μV, (44.8±2.4) ms (white); (104±39) μV, (47.8±0.8) ms (green); (86±33) μV, (47.2±1.9) ms (blue) and (76±21) μV, (65.0±1.9) ms (red), respectively. The mERG in the light adaptation could also be recorded, but the amplitude was smaller. The cone cell degeneration rat had normal scoptic ERGs and OPs, but the photopic ERG disappeared. In the light adaptation achromatic mERG was extinguished and mERG waves were hard to recognize in the dark adaptation. But the chromatic mERG could be recorded in dark adaptation. The total responses were (42±7) μV, (50±7) ms (green); (31.1±0.4) μV, (50±8) ms (blue); and (125±41) μV, (57±3) ms (red), respectively. The congenital stationary night blindness rats had abnormal scoptic ERG and mERG. The achromatic mERG b waves declined and latency delayed compare with normal rats and the rats with cone cells degeneration. CONCLUSION The achromatic and chromatic mERGs could be recorded in normal rats cornea. The characteristic of waves is related to the colors. One case of congenital cone cells degeneration rat was found. It had abnormal photopic ERG and mERG. It was sensitive to red light stimulation on mERG recording model in the dark adaptation.
出处
《第四军医大学学报》
北大核心
2002年第11期986-989,共4页
Journal of the Fourth Military Medical University
