摘要
目的 探讨细胞因子白介素 18(IL 18)对大肠癌细胞Fas配体 (FasL)蛋白表达水平的调控 ,及其对肿瘤细胞反向杀伤T淋巴细胞作用的影响。方法 应用Western蛋白印迹法 ,检测IL 18作用后的人大肠癌SW 6 2 0细胞FasL蛋白表达水平的变化 ;应用流式细胞术分析IL 18处理后肿瘤细胞所诱导的Jurkat淋巴细胞凋亡率的变化。结果 10 μg/L和 10 0 μg/L两种浓度的IL 18作用 9h后 ,SW6 2 0细胞FasL蛋白开始上调 ,分别于作用 18和 36h后达到峰值 ,持续 72h后 ,FasL表达水平恢复到初始状态。随着FasL表达水平的上调 ,肿瘤细胞所诱导的Jurkat细胞凋亡率上升。结论 细胞因子IL 18具有上调大肠癌细胞FasL蛋白表达水平 。
Objective To investigate the modulation of FasL protein in colon cancer cells by interleukin 18 (IL 18) and it's effect on tumor cells counterattacking T lymphocytes. Methods By using Western blotting assay, FasL protein expression was examined in human colon cancer cell line SW620. The ratio of apoptosis of lymphocytes induced by tumor cells was studied by flow cytometry analysis. Results 10 μg/L and 100 μg/L IL 18 upregulated FasL protein expression in SW620 cell line, reaching the peak in 18 and 36 hours respectively, and returned to initial levels in 72 hours. The ratio of apoptosis of Jurkat cells induced by tumor cells increased in accordance with the upregulation of FasL protein. Conclusions IL 18 upregulates FasL protein expression in colon cancer cell line and enhances the ability of cancer cells to counterattack T lymphocytes.
出处
《中华消化杂志》
CAS
CSCD
北大核心
2002年第5期263-265,共3页
Chinese Journal of Digestion
基金
国家自然科学基金 (3 990 0 14 3 )
