摘要
目的 观察血管紧张素Ⅱ (AⅡ )对人单核细胞株THP 1分泌单核细胞趋化因子 (MCP 1)蛋白及基因的影响。方法 利用ELISA法检测AⅡ作用后THP 1分泌MCP 1的表达 ,利用RT PCR检测其MCP 1的mRNA表达。结果 4种不同浓度的AⅡ (1× 10 -6、1× 10 -7、1× 10 -8、1× 10 -9mol/L)刺激THP 12 4h后 ,MCP 1蛋白及mRNA的表达明显增加 ,且呈浓度依赖性。结论 AⅡ可调节THP 1细胞MCP 1基因及蛋白的表达 。
Objective Recent study suggests that atherosclerosis (AS) is a kind of inflammatory process . Although it is generally accepted that angiotensin Ⅱ(AⅡ) plays an important role in parthenogenesis ,the role of AⅡ in inflammation has not been explored. In this report ,we investigated the effect of AⅡ on the production of MCP 1 in THP 1 cells. Methods Cultured THP 1 was incubated with AⅡ(from 10 -9 to 10 -6 mol/L) for various length of time. MCP 1 was measured in THP 1 supernatants by ELISA . MCP 1 mRNA was detected by RT PCR. Results AⅡ significantly increased the expression of MCP 1 protein in a dose dependent manner and AⅡ also induced the expression of mRNA.Conclusions AⅡ may contribute to atherogenesis by activation of MCP 1 before the level of translation.
出处
《免疫学杂志》
CAS
CSCD
北大核心
2002年第4期278-280,共3页
Immunological Journal
基金
国家卫生部自然科学基金资助项目 (96 2 2 79)
