摘要
目的 :比较计算机辅助精液分析与常规精液分析的各项参数结果 ,用于临床精子质量的评估。方法 :对2 7例生育男性及 317例不育男性患者采用计算机辅助精液分析 (CASA)与常规精液分析 (RSA)。结果 :与 RSA比较 CASA存在精子畸形率降低 (CASA生育组 2 5 .10± 8.5 7、不育组 31.4 8± 12 .81,RSA生育组 35 .5 6± 9.5 8、不育组 5 4.5 6± 13.4 7) P<0 .0 1;生育组活率 CASA6 1.35± 7.6 2、RSA83.96± 6 .6 7,活力 A、B级精子降低 (CASA9.10± 5 .5 0、31.16± 12 .35 ,RSA30 .33± 8.85、4 9.93± 8.5 6 ) P<0 .0 1;活力 C、D级精子上升 (CASA2 1.0 6±14 .19、38.6 6± 7.6 2 ,RSA3.70± 2 .12、16 .0 4± 6 .6 7) P<0 .0 1;不育组活率无差异时 (CASA4 2 .5 3± 14 .95、RSA4 3.0 9± 13.96 ) ,活力 B级精子降低 (CASA2 2 .6 7± 14 .96、RSA33.32± 13.4 7) ,C级精子上升 (CASA17.0 8±13.4 7、RSA7.6 8± 5 .82 ) ,两者分析均有非常显著性差异 P<0 .0 1;非前向性运动组有 B级精子 CASA2 .77±6 .0 1、RSA0。另外 CASA还增设了直线速度、曲线速度、平均路径速度、精子侧摆幅度、精子轨迹、静态图等。结论 :计算机辅助精液分析对精子形态、活力辨别能力较常规精液分析低 ,但计算机辅助精液分析增设的量?
Objective: To compare computer assisted sperm analysis (CASA) with routine sperm analysis (RSA) in evaluation of male fertility. Methods: The results of CASA were compared with the RSA of 27 males normal fertility and 317 males with infertility. Results: Using the CASA, deformity rate was lower than RSA,it was 25.10±8.57 and 31.48±12.81 in the fertile and infertile groups respectively,whereas, RSA was 35.56± 9.58 and 54.56±13.47.Fertile group motility had a significant difference, it was 61.35±7.62 and 83.96± 6.67 . Sperm vitality of grades A was 9.10±5.50 and 30.33±8.85.The vitality of grades B was 31.16±12.35 and 49.93±8.56,the vitality of grades C was 21.06±14.19 and 3.70±2.12,the vitality of grades D was 38.66±7.62 and 16.04±6.67.When two techniques were adopted, the mobility had no difference in infertile group(42.53±14.95 and 43.09±13.96),the sperm vitality was decreased in grades B (CASA 22.67±14.96?RSA 33.32±13.47),increased in grades C(CASA 17.08±13.47?RSA 7.68±5.82),they had significant differences( P <0.01).The grade B sperms in the non forward moving group, CASA was 2.77±6.01, RSA 0.In addition, VSL,VCL,VAP,ALH, sperm trace and static diagram were adopted. Conclusion: Using the CASA, the distinguished ability to sperm shape and vigor was lower than RSA, but the quantize marker(such as straight line velocity, curved velocity, average path velocity and ALH) and moving, static images could reflect on sperms quality more objectively.
出处
《浙江大学学报（医学版）》
CAS
CSCD
2002年第3期215-218,共4页
Journal of Zhejiang University(Medical Sciences)
