摘要
目的 :探讨超排卵对瘦素水平的影响以及瘦素水平对体外受精 -胚胎移植 (IVF- ET)结局的影响。方法 :应用酶联免疫吸附法和放射免疫法分别测定 39例 IVF- ET患者在注 FSH、HCG前和取卵前的血清瘦素及排卵前卵泡的卵泡液瘦素、E2 水平。结果 :血清瘦素水平在注 HCG前 (2 6 .1± 2 .3)μg/ L明显高于注 FSH前 (9.3± 1.0 )μg/ L和取卵前 (15 .8± 2 .3) μg/ L,P均 <0 .0 0 1;卵泡液 (18.5± 2 .2 ) μg/ L和同期血清 (15 .8± 2 .3) μg/ L的瘦素水平比较 ,经 t检验无显著性差异 ;妊娠组的卵泡液和同期血清瘦素水平 (11.7± 1.8) μg/ L和 (8.7± 1.5 ) μg/ L明显低于非妊娠组的卵泡液和同期血清瘦素水平 (2 0 .8± 2 .7) μg/ L和 (18.3± 2 .9) μg/ L,P<0 .0 5。结论 :超排卵后瘦素浓度过高可能不利于卵泡的生长和发育。
Objective: To investigate the impact of ovarian stimulation on leptin levels and their effect on in vitro fertilization embryo transfer (IVF ET). Methods: In 39 women who underwent IVF ET, serum and follicular fluid leptin and E 2 levels were measured by enzyme linked immunosorbent assay and radioimmunoassay. Blood samples were collected just prior to FSH and HCG injection and follicles puncture. Follicular fluid was collected at the time of oocyte retrieval. Results: The serum leptin levels before HCG administration (26.1±2.3)μg/L were significantly higher than before FSH administration (9.3±1.0 )μg/L and oocyte retrieval (15.8±2.3)μg/L ( P < 0.001 ). On the day of oocyte retrieval, there was no significant difference between the serum leptin (15.8±2.3)μg/L and the follicular fluid leptin levels(18.5±2.2)μg/L. Serum and follicular leptin levels in successful pregnancies were significantly lower( P <0.05) than in women with failed conception. Conclusion: It could indicate that high leptin levels may interfere with the developing of dominant follicles.
出处
《浙江大学学报（医学版）》
CAS
CSCD
2002年第3期155-158,共4页
Journal of Zhejiang University(Medical Sciences)
关键词
瘦素
超排卵
体外受精-胚胎移植
影响
Leptin
Fertilization in vitro
Embryo transfer
Pregnancy
Superovulation
