摘要
目的 观察原发性肾病综合征 (PNS)患者细胞免疫功能变化 .方法 采用双抗体夹心 EL ISA法检测血清可溶性白细胞介素 2受体 (s IL - 2 R) ,流式细胞仪测定外周血 T细胞亚群 (CD3+ ,CD4 + ,CD8+ )及红细胞 C3b受体花环 (RBC-C3b RR)和红细胞免疫复合物花环 (RBC- ICR)试验 .结果 原发性肾病综合征组 s IL - 2 R明显高于对照组 [(398± 12 2 ) vs(2 0 2± 84 ) pm ol· L- 1 ,P<0 .0 1],T细胞亚群 CD3+ ,CD4 + ,CD8+较对照组明显降低 (0 .5 0± 0 .0 8) vs(0 .71±0 .0 7) ,(0 .2 6± 0 .0 7) vs(0 .36± 0 .0 7) ,(0 .2 2± 0 .0 5 ) vs(0 .31± 0 .0 6 ) ,P<0 .0 1,CD4 + / CD8+ 较对照组明显降低[(1.12± 0 .2 2 ) vs (1.32± 0 .30 ) ,P<0 .0 5 ],RBC- C3b RR较对照组明显降低 [(14 .5 %± 3.7% ) vs(2 1.4 %± 2 .3% ) ,P<0 .0 1],RBC- ICR与对照组比较明显增高 [(17.8%±5 .2 % ) vs(8.8%± 2 .1% ) ,P<0 .0 1].
AIM To investigate cellular immune function anderythrocyte immune function in patients with primary nephrotic syndrome (PNS). METHODS Soluble interleukin 2 receptors (sIL 2R) were detected by ELISA. T lymphocyte subsets (CD3 +, CD4 + and CD8 +) were also evaluated by flow cytometry (FCM). The red blood cell C 3b receptor rosette (RBC C 3b RR) and the red blood cell immune complexs rosette (RBC ICR) were measured. RESULTS Serum sIL 2R levels were significantly higher in PNS than in normal controls [(398 ±122) vs (202±84) pmol·L -1 , P < 0.01)], CD3 +, CD4 +, CD8 + were markedly decreased [ (0.50± 0.08) vs (0.71±0.07), (0.26±0.07) vs (0.36±0.07), (0.22±0.05) vs (0.31±0.06)] respectively, ( P <0.01). CD4 +/CD8 + ratios were lower than that in normal controls [(1.12±0.22) vs (1.32±0.30), P <0.05)]. RBC C 3b RR were significantly decreased [(14.5%±3.7%) vs (21.4%±2.3%), P <0.01)]. RBC ICR were significantly increased [(17.8%±5.2%) vs (8.8%±2.1%), P < 0.01)]. CONCLUSION Abnormal immune function may be important in the pathogenesis of PNS.
出处
《第四军医大学学报》
北大核心
2002年第12期1118-1120,共3页
Journal of the Fourth Military Medical University
