摘要
目的 探讨188Re直接法标记生长抑素类似物RC 16 0的方法及观察其在小鼠体内的生物学分布。方法 酒石酸亚锡直接还原法进行RC 16 0的188Re标记 ,标记完成后加入抗坏血酸以防标记产物的再氧化。硅胶纸层析测定放化纯 ,并行小鼠体内分布实验。结果 188Re RC 16 0放化纯为 96 .2 % ,游离188Re为 0 .8% ,放射性胶体为 3.0 %。加抗坏血酸组在标记后 2 4h放化纯为 85 % ,对照组为 5 0 %。注射后 0 .5～ 1.0h血液中放射性下降了 87.2 % ,肾脏无明显浓集 ,标记物 2 4h内由消化系统排出体外。结论 188Re直接法标记RC 16 0放化纯高 ,方法简便 ,抗坏血酸的加入增加了标记物的稳定性。188Re RC 16 0在小鼠体内血液清除较快 ,经消化系统排除。
Objective To investigate labeling method of RC-160 with 188 Re and the biodistribution of 188 Re-RC-160 in mice. Methods RC-160 was labeled with 188 Re through stannous tartrate reduction at room temperature under nitrogen gas condition. Ascorbic acid was added to inhibit re-oxidation of 188 Re-RC-160. The radiochemical purity was tested with silicon paper chromatography. Its biodistribution in normal mice was analyzed. Results The radiochemical purity was 96.2%. Free 188 Re was less than 0.8%. Radioactive colloid was 3%. The radiochemical purity at 24 h after labeling was 85% in ascorbic acid group while 50% in control group. At 0.5～1.0 h after injection, radioactivity in blood decreased by 87.2%. No obvious accumulation of 188 Re-RC-160 in the kidney was observed. A great amount of radioactivity was detected in gastrointestinal system within 12～24 h. Conclusions The method is simple and easy to perform. The radiochemical purity is quite high. The stability of 188 Re-RC-160 is improved by adding ascorbic acid. Blood clearance of 188 Re-RC-160 in normal mice is quite fast. 188 Re-RC-160 is excreted through gastrointestinal tract.
出处
《中华核医学杂志》
CAS
CSCD
北大核心
2002年第3期177-178,共2页
Chinese Journal of Nuclear Medicine
基金
国家自然科学基金资助项目 ( 39870 2 18)
全军医药卫生科研基金资助项目 ( 0 1MB12 0 )
