摘要
本文将样品直接皂化、环己烷提取,用分配柱和吸附柱进行提取液的净化和浓缩,HPLC法测定了冬虫夏草及虫草乌鸡胶丸中麦角甾醇的含量,为虫草及其制剂中麦角甾醇的含量测定建立了简便可行的方法.
high performance liquid chromatographic method(HPLC)was devel-oped for the determination of ergosterol in Cordyceps sinensis and Cordyceps black-bone chicken capsules.The sample was mixed with 0.5 ml of saturated KOH solution and 5 ml of ethanol,and refluxed for 1 h.Thc saponified mixture was diluted with 5 ml of 10%NaCl solution and extracted 3 times with 5 ml portions of cyclohexane.The cyclohexane extracts were purified by partition column and concentrated by adsorption column and then analysed by HPLC.The other conponents of the Cordyceps black-bone chicken cap sules have no interference to the determination of ergosterol.The proposed method is rap id.precise,accurate and sensitive.The whole process can be done in 2 h.
作者
李云华
李修禄
YH Li;XL Li(School of Phumacy,Secona Milttary Meduul Unrveratty,Shanghai 200433)
出处
《药学学报》
CAS
CSCD
北大核心
1991年第10期768-771,共4页
Acta Pharmaceutica Sinica
关键词
高效液相色谱法
麦角甾醇
冬虫夏草
蛹虫草
人工虫草
虫草乌鸡胶丸
HPLC
Ergosterol
Cordycepssinensis
Cordyceps militaris
Cultured Cordyceps
Cordycepsblack-bone chicken capsule
