摘要
本文用超临界流体色谱法(SFC)测定了三七和云南白药中人参二醇及人参三醇的含量。使用15%硫酸乙醇—水(1:1)直接进行水解,然后碱水解,克服了文献报道的水解不完全、结果不稳定、不能定量回收的缺点。本法取样量少,灵敏度高,操作简单、快速、整个分析过程可在8h内完成。
Capillary supercritical fluid chromatography(SFC)was developed for the determination of panaxadiol and panaxatriol in Radix notoginseng and Yunnan Baiyao.0.1 g Radix notoginseng powder or 0.5 g Yunnan Baiyao was mixed with 10 ml 15%HSOethanol-water(1:1)solution,adding 1 mg cholesterol as internal standard.The mixture was refluxed for 4 h,then adding 15 ml 15%NaOH solution,refluxed for 0.5 h.The mixture was extracted 3 times with 10 ml portions of cyclohexane.The cyclohexane extracts were purified by partition column and concentrated by adsorption column and then analysed by SFC.The proposed method is sensitive,accurate,precise,simple and rapid;all the process can be done in 8 h.
作者
李云华
李修禄
虹岚
刘锦耀
张美义
YH Li;XL Li;L Hong;JY Liu;MY Zhang(School of Pharmacy,Second Military Medical University,Shanghai 200433;Analytical and Testing Research Institute of Yunnan,Kunming 650011;Pharmacy,43th Hospital,Kunming 650032)
出处
《药学学报》
CAS
CSCD
北大核心
1991年第10期764-767,共4页
Acta Pharmaceutica Sinica
基金
国家自然科学基金资助项目
关键词
超临界流体色谱
人参二醇
人参三醇
三七
云南白药
Supercritical fluid chromatography
Panaxadiol
Panaxatriol
Radix notoginseng
Yunnan Baiyao
