摘要
以表面活性剂的胶束溶液为流动相的胶束色谱技术已引人重视,并已成功地应用于药物的薄层色谱分离鉴定。黄酮类化合物是中草药中广泛存在的一类有效成分。本文以十余种表面活性剂的胶束溶液为流动相研究了槲皮素、桑色素和芦丁的胶束薄层色谱行为,拟出了以4％Zeph—乙酰丙酮—水(5:1.5:1.5)溶液为流动相,聚酰胺薄膜为固定相分离此三种黄酮的层析体系,并对槐花进行分离和鉴定,得到了良好效果。
The micellar thin layer chromatographic behaviours of rutin, quercetin and morin were studied. The methods of separation and identification were established:Polyamide thin coating was used as the stationary phase and 4% Zeph- acetylacetoneH_2O (5:1.5:1.5) used as the mobile phase. The method was applied to the separation and identification of quercetin and rutin in samples of Sophorae flos and satisfactory result was obtained.
出处
《药学学报》
CAS
CSCD
北大核心
1991年第6期471-474,共4页
Acta Pharmaceutica Sinica
基金
国家自然科学基金
关键词
胶束薄层色谱
表面活性剂
黄酮
Micellar TLC
Surfactants
Quercetin
Morin
Rutin
