摘要
本文用GC/MS方法,对人尿中脱氢睾酮(boldenone)代谢物进行了研究。志愿者口服20 mg脱氢睾酮后,收集阳性尿。尿样经过包括XAD-2树脂柱吸附、酶水解、有机相萃取及三甲基硅烷衍生化反应的预处理后,用毛细管气相色谱与质谱检测器联用进行分析,鉴定了脱氢睾酮的几个主要代谢物和它们的代谢模式;总结了兴奋剂检测中具有意义的代谢物及其碎片离子;对收集的阳性尿中脱氢睾酮浓度进行测定;对预处理方法的回收率进行了研究。
The metabolism of boldenone (17β - hydroxy - 1,4 - androstem - 3 -one) in man has been investigated by gas chromatography/mass spectrometry. After oral administration of a 20 mg dose to man, six metabolites were detected in the conjugated fraction of the urinary samples. Boldenone, the major compound excreted in urine, was detected within 34 h after administration.In addition , several metabolites, resulting from the hydroxylation of boldenone and the reduction of the unsaturated carbon bonds of boldenone,were detected in the urine samples varying from 9 to 83 h. Extraction and fractionation of these metabolites were achieved by using XAD- 2 column and gas chromatography. The recovery of the whole procedure was studied. Furthermore, the mass spectra of the metabolites are presented and major fragment pathways are discussed.
出处
《药学学报》
CAS
CSCD
北大核心
1991年第5期362-366,共5页
Acta Pharmaceutica Sinica
