摘要
本文介绍用乳化—冻凝技术制备甲氨蝶吟—明胶微球的方法。实验结果证实,包裹在微球内的MTX对^(60)钻幅射、温度和光照射是稳定的。微球的体外溶出试验、明胶微球在介质中不同时间的溶胀度试验也在文中介绍。微球肝动脉栓塞实验治疗用大鼠移植性肝癌进行,结果表明MTX微球治疗组的大鼠在肿瘤抑制率、促使肿瘤坏死程度以及延长荷瘤动物存活期方面比肝动脉灌注生理盐水、MTX溶液和明胶微球为佳.由于MTX微球具有阻断肿瘤血供和在其局部缓释化疗药物等双重功用,故治疗肝癌的效果明显优于动脉化疗或单纯栓塞方法。
Preparation of methotrexate microsphere ( MTX-ms) by emulsion-freezing technique was introduced and the experimental results proved that MTX entrapped in the microspheres exhibited good stabilties towards temperature, cobalt- 60 radiation and light. The dissolution and inflation rate of the microspheres in pH 7.4 buffer solution at different times measured by Coulter counter was presented.Antitumor activity of MTX - ms after hepatic arterial embolization was examined in a model of liver tumor in Wistar rats. The group of rats treated with MTX-ms showed a rather significant reduction in tumor growth and more extented tumor necrosis as compared with the other groups, e. g. normal saline solution, MTX solution, placebo gelatin- ms and the results demonstrate that the effect of arterial chemoembolization used by MTX-ms is superior to that of the groups either using arterial chemotherapy or arterial embolization alone in treating rat liver cancer.
出处
《药学学报》
CAS
CSCD
北大核心
1991年第4期293-298,共6页
Acta Pharmaceutica Sinica
关键词
微球
肝动脉栓塞
甲氨蝶呤
Microsphere
Hepatic arterial embolization
Methotrexate
