摘要
对云南省的唐菖蒲花叶病进行调查和病样采集 ,通过鉴别寄主反应、电镜负染观察、免疫双扩散实验证实此病原为黄瓜花叶病毒CMV .
The samples of Gladiolus mosaic were invested and collected from in Yunnan Province. The isolate of Gladiolus mosaic can caused mosaic on Nicotiana glutinasa,N.tobacum,N.rustica,N.clevelandii,Cucumis sativus ; caused local lesions on Phaseolus vulgaris,Vigna sinensis,Chenopodium amarantidor,C.quinoa . The spherical virus particals with Ф?28～30?nm were detected under EM, with native stain method. The virus showed viral charactstic UV absorbing spectrum, A260/280=1.78. It reacted with CMV antiserum. From all above, it seems reasonable to conclud that the isolate is one stain of CMV.
出处
《云南农业大学学报》
CAS
CSCD
2002年第2期147-149,共3页
Journal of Yunnan Agricultural University
基金
云南省院省校合作研究项目 (98y90 0 9)
