摘要
为了观察马杜霉素对球虫超微结构的影响 ,探讨马杜霉素抑杀球虫的作用机理 ,将马杜霉素按0 .0 0 0 5%浓度混入鸡的育雏饲料中 ,1 2日龄时 ,用该饲料喂给药组的鸡 ,同时 ,另设定 1组不用药对照组 ,在1 4日龄时 ,参加实验的 2个组的每只实验鸡接种对马杜霉素敏感的孢子化的鸡柔嫩艾美尔球虫卵囊 2× 1 0 4个 ,感染后 84,96,1 0 8,1 2 0和 1 32 h每组分别捕杀 2只实验鸡 ,取鸡盲肠组织 ,制成超薄切片 ,通过透射电镜观察鸡体内的柔嫩艾美耳球虫敏感株在马杜霉素作用下超微结构的变化 ,与对照组比较 ,发现线粒体表现为数量增多和形态改变。形态改变表现在有的线粒体弯曲为马蹄形或者弓形 ;内部结构的变化为 :有的线粒体内膜和嵴脱落 ,内部形成空泡、膜状结构、螺旋状或者髓鞘样结构 ,有的线粒体的嵴变为与线粒体的长轴平行排列 ,且贯穿线粒体。作者推测马杜霉素等抗生素类抗球虫药的作用机理为 :破坏虫体内部结构与抑制虫体的生理机能
A strain of Eimeria tenella , which is sensitive to maduramasin, is treated with 5×10 -6 maduramasin in 14 day old chickens. The change of mitochondrion of the strain is observed and described. The number of the mitochondrion increased. The shape and structure of the mitochondrion changed. The mitochondrion swelled into round. Some mitochondria turned into banana shape. The cristae of the mitochondrion turned into paralleling to the longitudinal axis and run through it. The inner membrane and cristae separated from the eternal membrane, and then turned into vacuole. The marrow sheathed,electric dense figures and membrane figures were found in the mitochondrion. The mechanism that maduramasin inhibit or kill Coccidia is attributed to the destructure of the structure and function.
出处
《中国农业大学学报》
CAS
CSCD
北大核心
2002年第3期118-122,共5页
Journal of China Agricultural University
基金
国家科委"八五"攻关资助项目 (91 0 4 1 1 4 38)
