摘要
目的 :调查胃癌患者p16基因的改变。方法 :采用PCR和DNA测序方法 ,对 36例胃癌患者肿瘤组织标本的p16基因外显子 1、2和 3进行检测 ,研究p16基因的缺失和突变情况。结果 :发现 2例有p16基因外显子 1的纯合缺失 ,3例有外显子 3的纯合缺失 ,缺失频率为 13 89% ,5例均为弥漫型胃癌。所有标本未检出点突变。结论
AIM: To investigate p16 gene alterations in gastric carcinoma. METHODS: 36 fresh tumor specimens taken from gastric cancer patients were analyzed for p16 gene deletion and mutation by polymerase chain reaction (PCR) and DNA sequencing. RESULTS: Homozygous deletion of exon 1 and exon 3 was observed in 2 cases and 3 cases of diffuse carcinoma, respectively. The frequency of homozygous deletion was 13.89%. No p16 gene point mutation was detected. CONCLUSION: The deletion of p16 genes may be related to gastric carcinogenesis.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2002年第5期543-545,共3页
Chinese Journal of Pathophysiology
基金
安徽省教委自然科学基金资助 (No .99jl0 2 16 )
关键词
胃癌
P16基因
纯合缺失
基因突变
Stomach neoplasms
Genes, p16
Homozygous deletion
Gene mutation
