摘要
观察雌激素受体在雌性与雄性大鼠心脏中的表达。取大鼠心房与心室组织制作冰冻切片 ,应用抗雌激素受体单抗进行免疫组织化学 (SP法 )染色并进行图像分析。结果显示 ,雌性与雄性大鼠心脏都存在雌激素受体 ,且受体的表达无性别差异 (P>0 .0 5 ) ;心房与心室都存在雌激素受体阳性表达 ,其表达也无明显差异 (P>0 .0 5 ) ;阳性反应见于心肌细胞和成纤维细胞。结果表明 ,大鼠心脏存在雌激素受体 ,心房与心室都可能是雌激素的靶组织 ;心血管疾病的性别差异与雌性、雄性的受体含量无关 。
To investigate the expression of estrogen receptor(ER) in the heart of rats, specimens of atria and ventricles were obtained from SD rats and made into frozen sections. The sections were examined with SP immunohistochemical technique and image analysis method. The results showed that the positive ER immunoreactivity existed in cardiac myocytes and fibroblasts of female and male rats' atria and ventricles. The expression of ER didn't have sexual differences and no differences between the atria and ventricles were observed either. These results suggested the heart of rat contains ER. The atria and ventricles probably are the target tissues of estrogen. The sexual diffenerces in the cardiovascular diseases aren't related to the content of ER. It may be involved in the state of activity and function of ER under the physiological conditions.\;
出处
《中国组织化学与细胞化学杂志》
CAS
CSCD
2001年第2期155-159,共5页
Chinese Journal of Histochemistry and Cytochemistry
基金
湖北省自然科学基金资助项目!(NO. 98J0 98)
关键词
性别差异
大鼠
心脏
雌激素受体
免疫组织化学
Estrogen
Estrogen receptor
Heart
Sexual difference
Immunohistochemistry
Rat
