摘要
本文介绍了一套简单快速的DNA银染以及胶保存的方法 ,整个过程仅需 10～ 15分钟 ,而且背景浅 ,条带清楚 ,灵敏度高 ,稳定性好。胶保存采用双层玻璃纸夹心法 ,可长久地保存胶显色时的原貌。以常规PAG胶检测和HLA的SSCP分型为例 ,利用该套方法进行了银染以及胶的保存 ,均得到了满意的结果。该方法具有推广价值。
This paper introduced the simple and rapid methods of silver staining and gel preservation.It was taken only about 10 and 15 minutes to stain a gel.The background of gel was light,the bands were clear,the sensibility was high and the stabilization was well by the method of silver staining.The gel preservation adopted a method named two-layer transparent plastic paper 'Sandwich' which could keep the gel with primitive colors for a long time.The methods were used on PAG checking and SSCP typing of HLA and the results were satisfactory.The set of methods are expected to be widely used in laboratories.
出处
《遗传》
CAS
CSCD
北大核心
2002年第3期335-336,共2页
Hereditas（Beijing)
基金
国家自然科学基金重大项目 ( 3999342 0 )
