摘要
目的观察腺病毒介导的自杀基因对消化系肿瘤的杀伤作用。方法腺病毒介导的自杀基因HSV-TK分别转染结肠癌LOVO细胞、胃癌MGL-803细胞和肝癌BEL-7402细胞,比较腺病毒载体对不同肿瘤细胞的转染效率。加入前药丙氧鸟苷(GCV),通过MTT法检测细胞存活率,观察单纯疱疹病毒1型胸苷激酶/丙氧鸟苷(HSV-TK/GCV)系统对不同肿瘤细胞的杀伤作用和旁观者效应。结果在GCV浓度100mg/L以上时,3种转基因肿瘤细胞均可被HSV-TK/GCV系统完全杀伤;旁观者效应以MGL-803细胞最为明显,与LOVO细胞和BEL-7402细胞相比,差异有显著性意义(P<0.05);腺病毒对肿瘤细胞的转染效率强弱依次为BEL-7402细胞、MGL-803细胞和LOVO细胞。结论重组腺病毒可以作为消化系肿瘤基因治疗的高效载体。HSV-TK/GCV系统对胃癌MGL-803细胞的作用最佳。
Objective To investigate antitumor effects of HSV TK gene with adenovirus mediation on alimentary carcinomas. Methods HSV TK gene was transfected to alimentary carcinoma cell lines (LOVO, MGL 803 and Bel 7402) mediated by recombinant adenovirus. Efficiency of transfection between each cell line was compared. Effect of killing tumor cell and bystander effect was observed after ganciclovir addition. Survival rate of tumor cells was detected by MTT. Results All transfected tumor cells could be killed at the dosage more than 100 mg/L of ganciclovir. Bystander effect was observed most obviously in MGL 803 cell as compared to LOVO and BEL 7402 cells (P< 0.05). BEL 7402, MGL 803 and LOVO were ranked in turn according to transfer efficiency of recombinant adenovirus. Conclusions Recombinant adenovirus can be used as high effective carrier applied in gene therapy of alimentary carcinoma. MGL 803 cell has the best response to Hsv tk/ganciclovir system.
出处
《中华胃肠外科杂志》
CAS
2002年第2期137-139,共3页
Chinese Journal of Gastrointestinal Surgery
基金
国家自然科学基金资助项目(39700145)
中山医科大学"211工程"重点学科建设基金资助项目(98095)
