摘要
目的 探讨抗氧化剂N 乙酰半胱氨酸 (NAC)对急慢性吸烟大鼠肺组织和肺泡灌洗液 (BALF)中核因子 κB (NF κB)表达的影响及机理。方法 将Wistar大鼠随机分成 :对照组 6只 ,吸烟组 30只 ,干预 (吸烟 +NAC)组 30只。其中吸烟组和干预组中动物均动态观察 1、 2、 7、 14、 6 0d (各时间点 6只 )的肺组织和BALF细胞中NF κB的激活水平。运用Western Blot免疫印迹电泳法 ,以抗NF κB抗体P5 0、P6 5作探针检测NF κB蛋白的表达量。结果 急慢性吸烟大鼠肺组织和BALF细胞中NF κB的表达以急性吸烟早期d 1、2达最高 ,肺组织在d 1、2的NF κB活性分别为 36 .9± 8.1和36 .9± 8.0 ,与对照组 7.3± 2 .8比较 ,均有显著性的差异 (P <0 .0 1) ;在d 1、2 ,BALF细胞的NF κB活性分别为 2 4 .0± 6 .1和 2 1.2± 5 .8,与对照组 5 .7±2 .6比较 ,也均有显著性的差异 (P <0 .0 1)。但吸烟7d ,NF κB的表达已有下降 ,并发现从急性吸烟 7、14d至慢性吸烟 6 0d ,NF κB维持在比较稳定的低水平表达 ,与对照组相比均无显著差异 (P >0 .0 5 )。NAC对被激活的NF κB有明显的抑制作用 ,与各自吸烟组相比P <0 .0 1,而对低水平NF κB表达的抑制作用不明显。结论 NAC通过抑制急性吸烟早期被激活的NF κB 。
Aim To assess the effect and mechanism of N acetylcysteine (NAC, antioxidant) on nuclear factor κB (NF κB) in lung tissue and bronhial alveolar lavage fluid(BALF) of rats undergoing acute and chronic smoking. Methods All Wistar rats were divided randomly into three groups: control group, smoking group and intervention group. The latter two groups were applied to observing NF κB expression during different periods (1, 2, 7, 14, 60d). Western Blot assay was used to analyze the activation of NF κB. Results The first and second day the expression of NF κB in lung tissue ( 36.9 ± 8.1 and 36.9 ± 8.0 )and in BALF( 24.0 ± 6.1 and 21.2 ± 5.8 ) rose significantly comparing with that in the control groups ( 7.3 ± 2.8 and 5.7 ± 2.6 )respectively (P< 0.01 ), but fell to the normal range of control at the seventh, fourteenth and sixtith days of smoking. NAC significantly inhibited the higher NF κB expression comparing with that in the related control smoking groups, but not inhibited the normal NF κB expression in the rest groups. Conclusion NAC, through inhibiting activation of NF κB during the early smoking stage and further blocking the activation of pro inflammatory cytokines, may play a role in pathogenesis of airway inflammation of COPD.
出处
《中国临床药理学与治疗学》
CAS
CSCD
2001年第2期103-106,共4页
Chinese Journal of Clinical Pharmacology and Therapeutics
