摘要
目的 :测定四倍体白芷药材中欧前胡素的含量 ,并与原二倍体白芷相比较。方法 :采用高效液相色谱法 ,色谱柱Nova pak 4 .6mm× 2 0 0mm ,5 μm ,流动相乙腈 水 ,流速 1mL·min-1,检测波长 2 4 8nm ,柱温 2 5℃。结果 :四倍体白芷药材中欧前胡素含量达 0 .4 6 % ,二倍体对照药材仅为 0 .2 2 5 % ,含量增加了 10 4 .4 %。结论
Objective: To determine the content of imperatorin in the tetraploidy radix angelicae dahuricae , and compare it with the original diplontic varites. Method: The chromatographic method was carried out on Nova pak (4.6 mm ×200 mm,5 μm ) column with acetonitrile water solution as a mobile phase. The flow rate was 1 ml·min -1 . the detection wavelength was at 248 nm, and the column temperature was 25 ℃.Result: The eontent of was imperatorin in the tetraploidy radix 0.460% and 0.225% imperatorin in the diplontic species. Conclusion: The content of the mainly active constituent in the tetraploid is double to what it is in the original diplontic species.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2002年第6期426-427,459,共3页
China Journal of Chinese Materia Medica
基金
湖南省科技厅资助项目 (97SSY10 0 5 )
