摘要
本文介绍了以亲和层析纯化的免抗鼠IgG类抗体及其酶标物作为捕获及检测抗体进行鼠杂交瘤培养上清及腹水中IgG类单克隆抗体的ELISA定量测定方法。结果表明:小牛血清(BS)、免血清(RS)、马血滑(HS)、人血清(HuS)及非抗体产生细胞的培养上清对本项εLISA实验无干扰,使测定的结果较可靠,测定的鼠IgG各亚类的标准曲线的线性范围均在5～100ng/ml之间,因此这个快速、可靠的方法可为鼠IgG类单克隆抗体的定量测定提供一种实用的手段。
An enzyme-linked immunosorbent assay (ELISA) has been investigated forqnantitating IgG monoclonal antibodies by using affinity-purified rabbit antimouse IgG antibody and its HRP conjugate for capture and detection. The results proved that the bovin serum, horse serum, rabbit serum, human serum and non-antibody producing cell culture super natants did not interfere with the ELISA. Therefore, the results were reliable . The linear ranges of standard curves of all mouse IgG subclasses were in the 5-100 ng/ml. This rapid and reliable assay may allow practically applicable in many situations in which quantiting murine monoclonal antibodies is needed.
出处
《厦门大学学报（自然科学版）》
CAS
CSCD
北大核心
1991年第4期433-437,共5页
Journal of Xiamen University：Natural Science
