摘要
目的 :采用HPLC法测定氯霉素地塞米松滴眼液中氯霉素和地塞米松磷酸钠的含量。方法 :采用C18色谱柱 ,流动相为甲醇 0 .0 2 5mol·L- 1的磷酸二氢钠溶液 (4 8∶52V/V) ,检测波长为 2 40nm。结果 :氯霉素的线性范围为 75～ 1 75μg·ml- 1,r=0 .9994,回收率 99.6 6 %。RSD =1 .0 3%;地塞米松磷酸钠的线性范围为 1 5～ 35μg·ml- 1;r =0 .9992 ,回收率 1 0 4.2 4%。结论 :该方法简便、准确 ,可同时测定滴眼液中氯霉素和地塞米松磷酸钠的含量。
OBJECTIVE To establish a method for simultaneous determination of chloramphenicol and dexa methasone sodium phosphate in eye drops by HPLC.METHODS Chloramphenicol and dexamethasone were eluted with methanal -0.025 mol·L -1 sodium dihydrogen phosphate solution(48∶52 v/v)on C 18 column and we re determined at 240 nm.RESULTS The linear range of chloramphenicol was 75～175 μg·ml -1 , r = 0.9994 ,the recovery was 99.66% ,RSD= 1.03% ;the linear range of dexamethasone s odium phosphate was 15～35 μg·ml -1 , r = 0.9992 ,the recovery was 104.24% ,RSD= 1.66% ?CONCLUSIONS The method is simple,accurate and suitable for the determination of chlorarnphe mlcol and dexamethasone sodium phosphate In eye drops.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2002年第6期334-335,共2页
Chinese Journal of Hospital Pharmacy
