摘要
目的:CagA和VacA能否作为幽门螺杆菌(H.pylori)相关性疾病的预测因子尚有争论,本文旨在研究胃癌高发区的福州市不同胃病患者的H.prLori CagA和VacA的检出率,探讨这二者作为H.pylori毒力标志物的可行性。 方法:胃镜或手术及病理证实的慢性胃病患者170例纳入研究,胃窦癌(CC)34例,十二指肠溃疡(DU)39例,胃溃疡(GU)35例,慢性胃炎62例。无症状的健康志愿者36名为对照组,免疫印迹法检测血清中H.pylori抗体。部分患者同时接受^(14)碳-尿素呼气检测。 结果:DU组、GU组和GC组的HP血清学阳性率高于胃炎组(X^2=4.84,P=0.028)和对照组(X^2=25.877,P<0.001),DU组与GU组、GC组之间的差异无显著性意义(X^2=3.306,P=0.191)。DU组的呼气实验阳性率高于GC组(X^2=16.463,P<0.001)、胃炎组(X^2=4.31,P=0.038)和对照组(X^2=33.33,P<0.001),GU组和胃炎组高于对照组(P<0.05);而DU组与GU组之间、GC组和对照组之间的差异均无统计学意义(P=0.144)。根据免疫印迹结果进行分型,各组间CgaA、VacA和Ⅰ型菌(CagA+、VacA+)阳性率无显著性差异(P>0.05)。 结论:尽管H.pylori感染与慢性胃病有关,但血清CagA和VacA抗体对预测H.pylori感染的后果没有意义。
AIM: It is controversial that CagA and VacA can predict the outcomes of Helicobacter pylori (H pylori) infection. This study was aimed to test the detected rate of CagA and VacA and then to assess their predictive values in some gastroduodenal diseases from patients in Fuzhou city, a region with higher prevalence of gastric cancer.METHODS: One hundred and seventy patients with chronic gastroduodenal diseases entered this study, including 34 patients with gastric antral cancer (GC), 39 duodenal ulcer (DU), 35 gastric ulcer(GU), and 62 chronic gastritis(CG). Thirty-six asymptomatic healthy volunteers were served as controls. Serum H. pylori antibodies (VacA, CagA and urease) were tested using immunoblot. 14 C-urea and breathing test was performed in some patients.RESULTS:Serum positive rates of H. pylori in DU, GU and GC groups were significantly higher than that in CG group (X2 =4.84, P = 0.028) and in healthy controls (X2 =25.877, P < 0. 001) respectively. There was no a significant difference among the DU, GU and GC groups (X2 = 3.306, P = 0. 191). According to 14 C-urea and breathing test,prevalence of H. pylori in the disease groups was significantly higher than that in healthy controls (P< 0.05), and that in DU group was higher than those in GC (X2 = 16. 463, P< 0.001), CG groups (X2 =4.31, P = 0.038) and healthy control (X2 =33.33, P<0.001) respectively. The positive rates of CagA and VacA antibodies and type I strain (CagA and VacA positive) were not different among the three disease groups (P> 0.05).CONCLUSION: Although H. pylori infection plays a certain role in the pathogenesis of gastroduodenal diseases, serum CagA and VacA antibody do not predict the outcome of this infection.
出处
《世界华人消化杂志》
CAS
2002年第5期533-535,共3页
World Chinese Journal of Digestology
基金
福建省三项费基金资助(99A036)
