摘要
目的探讨环境铅暴露对学龄前儿童免疫系统的影响。方法用石墨炉原子吸收光谱仪对217名学龄前儿童进行血铅测定 ;流式细胞仪检测T淋巴细胞亚群CD3+、CD3+CD4+、CD3 +CD8+以及B细胞 (CD3-CD19+)、NK细胞(CD3-CD16+CD56+)的分布 ;散射比浊法检测免疫球蛋白IgG、IgM水平 ;酶联免疫吸附试验 (ELISA)检测血清IgE水平。结果217名学龄前儿童血铅水平 :0.11～2.11μmol/L ,(0.46±0.27)μmol/L(x ±s) ,其中63名儿童血铅≥0.48μmol/L。选择高血铅组 (≥0.48μmol/L)38名和低血铅组 (<0.48μmol/L)35名儿童进行淋巴细胞亚群比较 ,高血铅组学龄前儿童CD3 +、CD3+CD4+/CD3+CD8+与低血铅组比较均明显下降 (P<0.01);CD3+CD8+细胞高血铅组与低血铅组相比升高 ,具有显著性意义 (P<0.05) ;CD3-CD19+、CD3-CD16+CD56+细胞、IgG、IgM、IgE高血铅组与低血铅组相比较差异无显著性 (P>0.05)。结论血铅≥0.48μmol/L对学龄前儿童T淋巴细胞亚群有不利影响。
Objective To explore the effects of environmental lead exposure on immune system in preschool children. Methods The blood lead levels of 217 preschool children were determined by graphite furnance atomic absorption spectrophotometry. The distribution of T_lymphocyte subsets: CD 3+,CD 3+CD 4+,CD 3+CD 8+, B cells (CD 3- CD 19+), NK cells (CD 3-CD 16+CD 56-) were analyzed by flow cytometer, the levels of serum immunoglobulin G and immunoglobulin M were determined by scattering turbidimetry, the levels of serum immunoglobulin E were examined with ELISA. Results The mean level of blood lead of 217 preschool children was (0.46±0.27)μmol/L(range:0.11～2.71 μmol/L). The blood lead levels of 63 preschool children were ≥0.48 μmol/L. 38 preschool children among 63 preschool children with blood lead level of ≥0.48 μmol/L were selected as the high_blood_lead group, 35 preschool children with blood lead levels of <0.48 μmol/L were served as low_blood_lead group. The comparative study on the distribution of T_lymphocyte subsets was carried out in these two groups. Lower percentage of CD 3+CD 4+cells, lower ratio of CD 3+CD 4+ cells to CD 3+CD 8+ cells, higher percentage of CD 3+CD 8+ cells were found in preschool children with blood lead level of ≥ 0.48 μmol/L compared with those of control group (P<0.01 or P<0.05). No significant differences were observed in the percentages of CD 3-CD 19+ cells, CD 3-CD 16+CD 56+ cells, and the levels of IgG, IgM, IgE between high_blood_lead group and control group (P>0.05). Condusion The blood lead levels of ≥0.48 μmol/L presented adverse effects on the T_lymphocyte subsets.
出处
《环境与健康杂志》
CAS
CSCD
北大核心
2002年第2期99-101,共3页
Journal of Environment and Health
基金
浙江省卫生厅基金资助项目 (2000A061)
关键词
铅中毒
儿童
学龄前
免疫
细胞
T细胞亚群
Lead poisoning
Children preschool
Immune, cell
T_lymphocyte subset
