摘要
采用间接酶联免疫法(ELISA)对棉花胚、花药和小麦旗叶的内源IAA、ABA、Z+ZR 含量测定,表明用ELISA 测定植物内源激素是可靠的。ELISA 能简化分离提取植物材料的步骤,节省供测材料的数量,提高被测物激素的收率,真实反映植物内源激素的水平。
A test where the indirect enzyme-linked immunosorent assay(ELISA)was used to determine the contents of IAA,Z+ZR and ABA in cottonembryos,cotton anthers and wheat flag leaves suggest that this techniqueis reliable for plant hormone estimation.Plant hormones can be measured.using only a small quantity of plant materials,the purification procedureis simpler,and the recovery rate is higher.
出处
《西南农业大学学报（自然科学版）》
CSCD
1991年第2期183-186,共4页
Journal of Southwest Agricultural University
关键词
植物激素
内源激素
ELISA
测定
phytohormone
endogenous hotmone
Dot-ELISA
cotton
triticum aestivum
