摘要
目的 :诱导兔骨髓间充质干细胞表达软骨细胞表型。方法 :抽取兔骨髓 ,经密度梯度离心和粘附分离得到MSCs。用生长因子诱导 ,考察细胞软骨特异性基质的表达水平。结果TGF β1,IGF I和维生素C结合可促进MSCs表达软骨特异性的Ⅱ型前胶原mRNA ,但并未促进成骨特异性的碱性磷酸酶合成和钙盐沉积。结论TGF β1,IGF I和维生素C结合诱导可促进MSCs增殖 ,表达软骨细胞表型 ,而不产生诱导成骨效应。
Objective: To establish a method of isolating rabbit bone marrow mesenchymal stem cells(MSCs) and inducing them to express chondrocyte phenotype.Method:Bone marrow was extracted from the iliac of New Zealand rabbit,MSCs were seperated by density centrifugation and fibronectin adhesion.TGF-β 1?IGF-I and Vitamin C were applied into culture medium to induce proliferation and transformation of MSCs.Procollagen α 1(Ⅱ) mRNA and ALP in culture were detected,von Kossa stain was carried out on cell creep slips to detect calcium deposit.Result:Cell proliferation and expression of articular cartilage matrix-procollagen α1(Ⅱ) mRNA were promoted by inducing factors-TGF-β 1?IGF-I and Vitamin C;at the same time,osteogenesis-specific ALP and calcium content deposition were not detected.Conclusion:Cell proliferation and expression of chondrocytic phenotype,but not osteoblastic phenotype,could be induced by the combination of TGF-β 1?IGF-I and Vitamin C.
出处
《中国矫形外科杂志》
CAS
CSCD
2002年第6期586-589,共4页
Orthopedic Journal of China
基金
陕西省自然科学基金项目 (2 0 0 1SM 79)
西安交通大学第一医院科研基金 (2 0 0 0YK0 2 )
关键词
骨髓间充质干细胞
细胞培养
软骨
Bone marrow
Mesenchymal stem cells
Cell culture
Cartilage
