摘要
将实验孕鼠随机分为5组,供实验的4个组于第7～18d 分别每天口服1.00、0.10、0.05及0.01mg/kg 敌枯双;对照组口服0.5%羧甲基纤维素钠。于孕20d 处死,取骨髓按常规细胞培养、镜检,发现各实验组的细胞畸变率均比对照组高,有高度显著性或显著性。说明其阈剂量在0.01mg/kg 以下,为一强的诱变剂。畸变的类型主要有断片、断裂、微粒、粉碎及多倍体等。
Pregnant rats were divided to 5 groups at random.From 7th to 18th days of pregnancy,4 groups of them were fed on Bis-A-TDA with the doses of 1.0,0.1,0.05,0.01mg/kg per day,1 group received 0.5% sodium car- boxy methyl fibrin as control.The rats sacrificed on 20th day.The bone marrow cells of the rats were cultured with the routine method and observed microscopically.It was found that the aberration rats of experimental groups were very significantly higher than that of the control one.So we concluded that the threshold dosage of Bis-A-TDA on the pregnant rats was less than 0.01mg/kg,and it was a strong mutagenic agent.The types of chromosomal aberratation were fragment,breakage,pulverization,minute fragment and multiploid.
关键词
骨髓
染色体
畸变
噻二唑
大鼠
thiadiazoles/TO
pesticides/TO
bone marrow/PA
chromosomal aberratation
rat
aminals
laboratory
